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Reconstitution of translocation-competent membrane vesicles from detergent-solubilized dog pancreas rough microsomes.

Dog pancreas rough microsomes were solubilized in 1% octyl beta-glucoside, and membrane vesicles were reconstituted by slow 30-fold dilution with a buffer of low ionic strength. Asymmetric assembly of the membranes occurred during reconstitution since the vesicles formed contained ribosomes bound on...

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Bibliografiske detaljer
Main Authors: Yu, Y H, Zhang, Y Y, Sabatini, D D, Kreibich, G
Format: Artigo
Sprog:Inglês
Udgivet: 1989
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC298616/
https://ncbi.nlm.nih.gov/pubmed/2602384
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