Reconstitution of translocation-competent membrane vesicles from detergent-solubilized dog pancreas rough microsomes.

Dog pancreas rough microsomes were solubilized in 1% octyl beta-glucoside, and membrane vesicles were reconstituted by slow 30-fold dilution with a buffer of low ionic strength. Asymmetric assembly of the membranes occurred during reconstitution since the vesicles formed contained ribosomes bound on...

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Vydáno v:	Proc Natl Acad Sci U S A
Hlavní autoři:	Yu, Y H, Zhang, Y Y, Sabatini, D D, Kreibich, G
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	National Academy of Sciences 1989
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298616/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2602384/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.24.9931
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