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Rapid empirical discovery of optimal peptides for targeted proteomics

We report a method for high-throughput, cost-efficient empirical discovery of optimal proteotypic peptides and fragment ions for targeted proteomics applications using in vitro-synthesized proteins. We demonstrate the approach using human transcription factors – which are typically difficult, low-ab...

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Detalhes bibliográficos
Main Authors: Stergachis, Andrew B., MacLean, Brendan, Lee, Kristen, Stamatoyannopoulos, John A., MacCoss, Michael J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3227787/
https://ncbi.nlm.nih.gov/pubmed/22056677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1770
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