A primer vector system that allows temperature dependent gene amplification and expression in mammalian cells: regulation of the influenza virus NS1 gene expression.

Influenza virus RNA segment 8 has been cloned into primer-vector pSLts1. This vector was designed to replicate in simian cells in a temperature dependent fashion by use of the SV40 tsA209 T-antigen gene. The oriented synthesis of cDNA on dT-tailed pSLts1 was performed on in vitro synthesized mRNA, a...

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Autores principales: Portela, A, Melero, J A, Martínez, C, Domingo, E, Ortín, J
Formato: Artigo
Lenguaje:Inglês
Publicado: 1985
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC322103/
https://ncbi.nlm.nih.gov/pubmed/2933634
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