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A primer vector system that allows temperature dependent gene amplification and expression in mammalian cells: regulation of the influenza virus NS1 gene expression.

Influenza virus RNA segment 8 has been cloned into primer-vector pSLts1. This vector was designed to replicate in simian cells in a temperature dependent fashion by use of the SV40 tsA209 T-antigen gene. The oriented synthesis of cDNA on dT-tailed pSLts1 was performed on in vitro synthesized mRNA, a...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nucleic Acids Res
Egile Nagusiak: Portela, A, Melero, J A, Martínez, C, Domingo, E, Ortín, J
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 1985
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322103/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2933634/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.22.7959
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