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A primer vector system that allows temperature dependent gene amplification and expression in mammalian cells: regulation of the influenza virus NS1 gene expression.
Influenza virus RNA segment 8 has been cloned into primer-vector pSLts1. This vector was designed to replicate in simian cells in a temperature dependent fashion by use of the SV40 tsA209 T-antigen gene. The oriented synthesis of cDNA on dT-tailed pSLts1 was performed on in vitro synthesized mRNA, a...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1985
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| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322103/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2933634/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.22.7959 |
| Oznake: |
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