Synthesis of phosphorothioate-containing DNA fragments by a modified hydroxybenzotriazole phosphotriester approach.

The phosphorothioylating agent which was obtained by treating 2,5-dichlorophenyl phosphorodichloridothioate with 1-hydroxy-6-nitrobenzotriazole proved to be very effective for the synthesis in solution and on a solid support of phosphorothioate-containing DNA fragments.

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Detalhes bibliográficos
Main Authors: Marugg, J E, van den Bergh, C, Tromp, M, van der Marel, G A, van Zoest, W J, van Boom, J H
Formato: Artigo
Idioma:Inglês
Publicado em: 1984
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC320440/
https://ncbi.nlm.nih.gov/pubmed/6549064
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