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Mechanism of the Orotidine 5’-Monophosphate Decarboxylase-Catalyzed Reaction: Importance of Residues in the Orotate Binding Site

The reaction catalyzed by orotidine 5’-monophosphate decarboxylase (OMPDC) is accompanied by exceptional values for the rate enhancement [k(cat)/k(non) = 7.1 × 10(16)] and catalytic proficiency [(k(cat)/K(M))/k(non) = 4.8 × 10(22) M(−1)]. Although a stabilized vinyl carbanion/carbene intermediate is...

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Détails bibliographiques
Auteurs principaux: Iiams, Vanessa, Desai, Bijoy J., Fedorov, Alexander A., Fedorov, Elena V., Almo, Steven C., Gerlt, John A.
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3199138/
https://ncbi.nlm.nih.gov/pubmed/21870810
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi2012355
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