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Rate-Limiting Steps and Role of Active Site Lys443 in the Mechanism of Carbapenam Synthetase

Carbapenam synthetase (hereafter named CPS) catalyzes the formation of the β-lactam ring in the biosynthetic pathway to (5R)-carbapen-2-em-3-carboxylate, the simplest of the carbapenem antibiotics. Kinetic studies showed remarkable tolerance to substrate stereochemistry on the turnover rate, but did...

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Detalhes bibliográficos
Main Authors: Arnett, Samantha O., Gerratana, Barbara, Townsend, Craig A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3198785/
https://ncbi.nlm.nih.gov/pubmed/17658887
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0618464
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