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Biochemical Characterization of Sfh-I, a Subclass B2 Metallo-β-Lactamase from Serratia fonticola UTAD54

The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBL...

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Detalhes bibliográficos
Main Authors: Fonseca, Fátima, Arthur, Christopher J., Bromley, Elizabeth H. C., Samyn, Bart, Moerman, Pablo, Saavedra, Maria José, Correia, António, Spencer, James
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2011
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3195014/
https://ncbi.nlm.nih.gov/pubmed/21876065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00429-11
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