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An NMR strategy for fragment-based ligand screening utilizing a paramagnetic lanthanide probe

A nuclear magnetic resonance-based ligand screening strategy utilizing a paramagnetic lanthanide probe is presented. By fixing a paramagnetic lanthanide ion to a target protein, a pseudo-contact shift (PCS) and a paramagnetic relaxation enhancement (PRE) can be observed for both the target protein a...

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Détails bibliographiques
Auteurs principaux: Saio, Tomohide, Ogura, Kenji, Shimizu, Kazumi, Yokochi, Masashi, Burke, Terrence R., Inagaki, Fuyuhiko
Format: Artigo
Langue:Inglês
Publié: Springer Netherlands 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3193990/
https://ncbi.nlm.nih.gov/pubmed/21927934
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-011-9566-5
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