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Peptide affinity reagents for AAV capsid recognition and purification
We report the discovery of AAV capsid-binding peptides identified through phage panning. The heptapeptide motif GYVSRHP selectively recognized AAV serotype 8 capsids and blocked transduction in vitro. Recombinant AAV8 vectors were purified directly from crude cell lysate and supernatant through sequ...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3192935/ https://ncbi.nlm.nih.gov/pubmed/21490687 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/gt.2011.46 |
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