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Imaging cytosolic NADH-NAD(+) redox state with a genetically encoded fluorescent biosensor
NADH is a key metabolic cofactor whose sensitive and specific detection in the cytosol of live cells has been difficult. We constructed a fluorescent biosensor of cytosolic NADH-NAD(+) redox state by combining a circularly permuted GFP T-Sapphire with a bacterial NADH-binding protein Rex. Although t...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3190165/ https://ncbi.nlm.nih.gov/pubmed/21982714 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cmet.2011.08.012 |
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