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The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.
M13 RF IV DNA may be prepared in vitro to contain phosphorothioate-modified internucleotidic linkages in the (-)strand only. Certain restriction enzymes react with this modified DNA to hydrolyze the (+)strand exclusively when a phosphorothioate linkage occurs at the normal cleavage point in the (-)s...
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| Main Authors: | , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1985
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318950/ https://ncbi.nlm.nih.gov/pubmed/3001650 |
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