The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.
M13 RF IV DNA may be prepared in vitro to contain phosphorothioate-modified internucleotidic linkages in the (-)strand only. Certain restriction enzymes react with this modified DNA to hydrolyze the (+)strand exclusively when a phosphorothioate linkage occurs at the normal cleavage point in the (-)s...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
1985
|
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318950/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3001650/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.24.8765 |
| תגים: |
אין תגיות, היה/י הראשונ/ה לתייג את הרשומה!
|