The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.
M13 RF IV DNA may be prepared in vitro to contain phosphorothioate-modified internucleotidic linkages in the (-)strand only. Certain restriction enzymes react with this modified DNA to hydrolyze the (+)strand exclusively when a phosphorothioate linkage occurs at the normal cleavage point in the (-)s...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1985
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318950/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3001650/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.24.8765 |
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