The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.

M13 RF IV DNA may be prepared in vitro to contain phosphorothioate-modified internucleotidic linkages in the (-)strand only. Certain restriction enzymes react with this modified DNA to hydrolyze the (+)strand exclusively when a phosphorothioate linkage occurs at the normal cleavage point in the (-)s...

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Udgivet i:Nucleic Acids Res
Main Authors: Taylor, J W, Ott, J, Eckstein, F
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1985
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318950/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3001650/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.24.8765
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