The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.
M13 RF IV DNA may be prepared in vitro to contain phosphorothioate-modified internucleotidic linkages in the (-)strand only. Certain restriction enzymes react with this modified DNA to hydrolyze the (+)strand exclusively when a phosphorothioate linkage occurs at the normal cleavage point in the (-)s...
Sparad:
| I publikationen: | Nucleic Acids Res |
|---|---|
| Huvudupphov: | , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
Oxford University Press
1985
|
| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318950/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3001650/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.24.8765 |
| Taggar: |
Inga taggar, Lägg till första taggen!
|