The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA.
The RF IV form of M13 DNA was synthesized enzymatically in vitro, using the viral (+)strand as template, to contain phosphorothioate-modified internucleotidic linkages of the Rp configuration on the 5' side of every base of a particular type in the newly-synthesized (-)strand. Twenty nine restrictio...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1985
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318949/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3001649/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.24.8749 |
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