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The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA.

The RF IV form of M13 DNA was synthesized enzymatically in vitro, using the viral (+)strand as template, to contain phosphorothioate-modified internucleotidic linkages of the Rp configuration on the 5' side of every base of a particular type in the newly-synthesized (-)strand. Twenty nine restr...

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Detalles Bibliográficos
Main Authors: Taylor, J W, Schmidt, W, Cosstick, R, Okruszek, A, Eckstein, F
Formato: Artigo
Idioma:Inglês
Publicado: 1985
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC318949/
https://ncbi.nlm.nih.gov/pubmed/3001649
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