The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA.

The RF IV form of M13 DNA was synthesized enzymatically in vitro, using the viral (+)strand as template, to contain phosphorothioate-modified internucleotidic linkages of the Rp configuration on the 5' side of every base of a particular type in the newly-synthesized (-)strand. Twenty nine restrictio...

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Bibliografiska uppgifter

I publikationen:	Nucleic Acids Res
Huvudupphov:	Taylor, J W, Schmidt, W, Cosstick, R, Okruszek, A, Eckstein, F
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	Oxford University Press 1985
Länkar:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318949/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3001649/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.24.8749
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