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The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA.
The RF IV form of M13 DNA was synthesized enzymatically in vitro, using the viral (+)strand as template, to contain phosphorothioate-modified internucleotidic linkages of the Rp configuration on the 5' side of every base of a particular type in the newly-synthesized (-)strand. Twenty nine restr...
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| Main Authors: | , , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1985
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318949/ https://ncbi.nlm.nih.gov/pubmed/3001649 |
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