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Site-specific cleavage of supercoiled DNA by ascorbate/Cu(II).

We have investigated ascorbate/Cu(II) cleavage of double-stranded DNA in the presence and absence of DNA negative torsion. We found that ascorbate/Cu(II) cleavage shows a site-specificity that is dependent on negative torsion and is influenced by the nature of the salt, ionic strength, and pH. This...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Wang, Y, Van Ness, B
Format: Artigo
Language:Inglês
Published: Oxford University Press 1989
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318422/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2780314/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.17.6915
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