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Site-specific cleavage of supercoiled DNA by ascorbate/Cu(II).

We have investigated ascorbate/Cu(II) cleavage of double-stranded DNA in the presence and absence of DNA negative torsion. We found that ascorbate/Cu(II) cleavage shows a site-specificity that is dependent on negative torsion and is influenced by the nature of the salt, ionic strength, and pH. This...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wang, Y, Van Ness, B
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1989
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC318422/
https://ncbi.nlm.nih.gov/pubmed/2780314
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