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Prevention of chain cleavage in the chemical synthesis of 2'-silylated oligoribonucleotides.

Strong aqueous ammonium hydroxide used to remove N-acyl protecting groups from synthetic oligoribonucleotides causes removal of some alkylsilyl protecting groups from 2'-hydroxyls and leads to chain cleavage. This problem is most severe when 30% ammonium hydroxide is used and substantially redu...

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Dettagli Bibliografici
Autori principali: Wu, T, Ogilvie, K K, Pon, R T
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1989
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC317792/
https://ncbi.nlm.nih.gov/pubmed/2726485
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