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Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy

Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic measurements of signaling events inside living cells, including detection of protein-protein interactions. An understanding of the basic physics of fluorescence lifetime measurements is required to use this technique....

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Autori principali: Sun, Yuansheng, Day, Richard N, Periasamy, Ammasi
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3169422/
https://ncbi.nlm.nih.gov/pubmed/21886099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.364
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