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Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy

Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic measurements of signaling events inside living cells, including detection of protein-protein interactions. An understanding of the basic physics of fluorescence lifetime measurements is required to use this technique....

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Hlavní autoři: Sun, Yuansheng, Day, Richard N, Periasamy, Ammasi
Médium: Artigo
Jazyk:Inglês
Vydáno: 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3169422/
https://ncbi.nlm.nih.gov/pubmed/21886099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.364
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