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Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy
Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic measurements of signaling events inside living cells, including detection of protein-protein interactions. An understanding of the basic physics of fluorescence lifetime measurements is required to use this technique....
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3169422/ https://ncbi.nlm.nih.gov/pubmed/21886099 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.364 |
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