Three-Color Spectral FRET Microscopy Localizes Three Interacting Proteins in Living Cells

FRET technologies are now routinely used to establish the spatial relationships between two cellular components (A and B). Adding a third target component (C) increases the complexity of the analysis between interactions AB/BC/AC. Here, we describe a novel method for analyzing a three-color (ABC) FR...

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Detalhes bibliográficos
Main Authors: Sun, Yuansheng, Wallrabe, Horst, Booker, Cynthia F., Day, Richard N., Periasamy, Ammasi
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2010
Assuntos:
Spectroscopy, Imaging, and Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2920763/
https://ncbi.nlm.nih.gov/pubmed/20713013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.06.004
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