IQGAP1 Interactome Analysis by In Vitro Reconstitution and Live Cell 3-Color FRET Microscopy

IQGAP1 stimulates branched actin filament nucleation by activating N-WASP, which then activates the Arp2/3 complex. N-WASP can be activated by other factors, including GTP-bound Cdc42 or Rac1, which also bind IQGAP1. Here we report the use of purified proteins for in vitro binding and actin polymeri...

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Detalhes bibliográficos
Main Authors: Wallrabe, Horst, Cai, Ying, Sun, Yuansheng, Periasamy, A., Luzes, R., Fang, Xiaolan, Kan, Ho-Man, Cameron, L. C., Schafer, Dorothy A., Bloom, George S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3917506/
https://ncbi.nlm.nih.gov/pubmed/24124181
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cm.21146
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