HEME-DEPENDENT ACTIVATION OF NEURONAL NITRIC-OXIDE SYNTHASE BY CYTOSOL IS DUE TO AN HSP70-DEPENDENT, THIOREDOXIN-MEDIATED THIOL-DISULFIDE INTERCHANGE IN THE HEME/SUBSTRATE BINDING CLEFT()

We have reported that heme-dependent activation of apo-neuronal nitric oxide synthase (apo-nNOS) to the active holo-enzyme dimer is dependent upon factors present in reticulocyte lysate and other cytosols. Here, we find that both Hsp70 and thioredoxin are components of the activation system. The apo...

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Bibliographic Details
Main Authors: Morishima, Yoshihiro, Lau, Miranda, Peng, Hwei-Ming, Miyata, Yoshinari, Gestwicki, Jason E., Pratt, William B., Osawa, Yoichi
Format: Artigo
Language:Inglês
Published: 2011
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3156863/
https://ncbi.nlm.nih.gov/pubmed/21755988
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi200751t
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