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Use of 2-Aminopurine Fluorescence To Study the Role of the β Hairpin in the Proofreading Pathway Catalyzed by the Phage T4 and RB69 DNA Polymerases

For DNA polymerases to proofread a misincorporated nucleotide, the terminal 3–4 nucleotides of the primer strand must be separated from the template strand before being bound in the exonuclease active center. Genetic and biochemical studies of the bacteriophage T4 DNA polymerase revealed that a prom...

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Hlavní autoři: Subuddhi, Usharani, Hogg, Matthew, Reha-Krantz, Linda J.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3153359/
https://ncbi.nlm.nih.gov/pubmed/18481871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi800211f
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