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Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms

High-throughput mRNA sequencing (RNA-Seq) holds the promise of simultaneous transcript discovery and abundance estimation(1-3). We introduce an algorithm for transcript assembly coupled with a statistical model for RNA-Seq experiments that produces estimates of abundances. Our algorithms are impleme...

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Detalhes bibliográficos
Main Authors: Trapnell, Cole, Williams, Brian A., Pertea, Geo, Mortazavi, Ali, Kwan, Gordon, van Baren, Marijke J., Salzberg, Steven L., Wold, Barbara J., Pachter, Lior
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3146043/
https://ncbi.nlm.nih.gov/pubmed/20436464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.1621
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