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TopHat: discovering splice junctions with RNA-Seq

Motivation: A new protocol for sequencing the messenger RNA in a cell, known as RNA-Seq, generates millions of short sequence fragments in a single run. These fragments, or ‘reads’, can be used to measure levels of gene expression and to identify novel splice variants of genes. However, current soft...

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Bibliografiske detaljer
Main Authors: Trapnell, Cole, Pachter, Lior, Salzberg, Steven L.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2672628/
https://ncbi.nlm.nih.gov/pubmed/19289445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/bioinformatics/btp120
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