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Improving RNA-Seq expression estimates by correcting for fragment bias
The biochemistry of RNA-Seq library preparation results in cDNA fragments that are not uniformly distributed within the transcripts they represent. This non-uniformity must be accounted for when estimating expression levels, and we show how to perform the needed corrections using a likelihood based...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3129672/ https://ncbi.nlm.nih.gov/pubmed/21410973 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2011-12-3-r22 |
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