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Improving RNA-Seq expression estimates by correcting for fragment bias

The biochemistry of RNA-Seq library preparation results in cDNA fragments that are not uniformly distributed within the transcripts they represent. This non-uniformity must be accounted for when estimating expression levels, and we show how to perform the needed corrections using a likelihood based...

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Détails bibliographiques
Auteurs principaux: Roberts, Adam, Trapnell, Cole, Donaghey, Julie, Rinn, John L, Pachter, Lior
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3129672/
https://ncbi.nlm.nih.gov/pubmed/21410973
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2011-12-3-r22
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