Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria—mini review

The advantages of phage Mu transposition-based systems for the chromosomal editing of plasmid-less strains are reviewed. The cis and trans requirements for Mu phage-mediated transposition, which include the L/R ends of the Mu DNA, the transposition factors MuA and MuB, and the cis/trans functioning...

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Autors principals: Akhverdyan, Valerii Z., Gak, Evgueni R., Tokmakova, Irina L., Stoynova, Nataliya V., Yomantas, Yurgis A. V., Mashko, Sergey V.
Format: Artigo
Idioma:Inglês
Publicat: Springer Berlin Heidelberg 2011
Matèries:
Mini-Review
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3145075/
https://ncbi.nlm.nih.gov/pubmed/21698377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-011-3416-y
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