Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome

A dual-component Mu-transposition system was modified for the integration/amplification of genes in Corynebacterium. The system consists of two types of plasmids: (i) a non-replicative integrative plasmid that contains the transposing mini-Mu(LR) unit bracketed by the L/R Mu ends or the mini-Mu(LER)...

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Bibliografiske detaljer
Udgivet i:Appl Microbiol Biotechnol
Main Authors: Gorshkova, Natalya V., Lobanova, Juliya S., Tokmakova, Irina L., Smirnov, Sergey V., Akhverdyan, Valerii Z., Krylov, Alexander A., Mashko, Sergey V.
Format: Artigo
Sprog:Inglês
Udgivet: Springer Berlin Heidelberg 2018
Fag:
Methods and Protocols
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5847225/
https://ncbi.nlm.nih.gov/pubmed/29392386
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-018-8767-1
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