Phage Mu-driven two-plasmid system for integration of recombinant DNA in the Methylophilus methylotrophus genome

A phage Mu-driven two-plasmid system for DNA integration in Escherichia coli genome has been adjusted for Methylophilus methylotrophus. Constructed helper plasmids with broad-host-range replicons carry thermo-inducible genes for transposition factors MuA and MuB. Integrative plasmids that are only r...

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Bibliografske podrobnosti
izdano v:Appl Microbiol Biotechnol
Main Authors: Abalakina, Elena G., Tokmakova, Irina L., Gorshkova, Natalya V., Gak, Evgueni R., Akhverdyan, Valerii Z., Mashko, Sergey V., Yomantas, Yurgis A. V.
Format: Artigo
Jezik:Inglês
Izdano: Springer Berlin Heidelberg 2008
Teme:
Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7419445/
https://ncbi.nlm.nih.gov/pubmed/18820908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-008-1696-7
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