Aromatic Amino Acid Auxotrophs Constructed by Recombinant Marker Exchange in Methylophilus methylotrophus AS1 Cells Expressing the aroP-Encoded Transporter of Escherichia coli

The isolation of auxotrophic mutants, which is a prerequisite for a substantial genetic analysis and metabolic engineering of obligate methylotrophs, remains a rather complicated task. We describe a novel method of constructing mutants of the bacterium Methylophilus methylotrophus AS1 that are auxot...

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Main Authors: Yomantas, Yurgis A. V., Tokmakova, Irina L., Gorshkova, Natalya V., Abalakina, Elena G., Kazakova, Svetlana M., Gak, Evgueni R., Mashko, Sergey V.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2798654/
https://ncbi.nlm.nih.gov/pubmed/19880640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02217-09
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