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Isolation of Auxotrophic Mutants of Methylophilus methylotrophus by Modified-Marker Exchange

A method for stabilizing a transposon (Tn5) has been developed which allows the isolation of stable auxotrophic mutants of Methylophilus methylotrophus ASI. Insertion of Tn5 into a cloned M. methylotrophus ASI DNA fragment encoding anthranilate synthase followed by transfer of the vector with the mo...

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Detalhes bibliográficos
Main Authors: Bohanon, Michael J., Bastien, Cathryn A., Yoshida, Roberta, Hanson, Richard S.
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC202433/
https://ncbi.nlm.nih.gov/pubmed/16347531
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