A combinatorial genetic library approach to target heterologous glycosylation enzymes to the Endoplasmic Reticulum or the Golgi apparatus of Pichia pastoris

To humanize the glycosylation pathway in the yeast Pichia pastoris we developed several combinatorial genetic libraries and used them to properly localize active eukaryotic mannosidases and sugar transferases. Here we report the details of the fusion of up to 66 N-terminal targeting sequences of fun...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Nett, Juergen H., Stadheim, Terrance, A., Li, Huijuan, Bobrowicz, Piotr, Hamilton, Stephen R., Davidson, Robert C., Choi, Byung-Kwon, Mitchell, Teresa, Bobrowicz, Beata, Rittenhour, Alissa, Wildt, Stefan, Gerngross, Tillman U.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2010
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3139792/
https://ncbi.nlm.nih.gov/pubmed/19908201
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.1731
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