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Generation and Screening of Pichia pastoris Strains with Enhanced Protein Production by Use of Microengraving
The selection of highly productive cell lines remains a key step for manufacturing therapeutic proteins. Microengraving was used to screen chemically mutagenized populations of Pichia pastoris for increased production of an Fc fragment. Clones retrieved following three rounds of mutagenesis yielded...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3126391/ https://ncbi.nlm.nih.gov/pubmed/21378037 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00104-11 |
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