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Generation and Screening of Pichia pastoris Strains with Enhanced Protein Production by Use of Microengraving
The selection of highly productive cell lines remains a key step for manufacturing therapeutic proteins. Microengraving was used to screen chemically mutagenized populations of Pichia pastoris for increased production of an Fc fragment. Clones retrieved following three rounds of mutagenesis yielded...
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| Главные авторы: | , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology
2011
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3126391/ https://ncbi.nlm.nih.gov/pubmed/21378037 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00104-11 |
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