A combinatorial genetic library approach to target heterologous glycosylation enzymes to the Endoplasmic Reticulum or the Golgi apparatus of Pichia pastoris

To humanize the glycosylation pathway in the yeast Pichia pastoris we developed several combinatorial genetic libraries and used them to properly localize active eukaryotic mannosidases and sugar transferases. Here we report the details of the fusion of up to 66 N-terminal targeting sequences of fun...

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Detalhes bibliográficos
Main Authors: Nett, Juergen H., Stadheim, Terrance, A., Li, Huijuan, Bobrowicz, Piotr, Hamilton, Stephen R., Davidson, Robert C., Choi, Byung-Kwon, Mitchell, Teresa, Bobrowicz, Beata, Rittenhour, Alissa, Wildt, Stefan, Gerngross, Tillman U.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3139792/
https://ncbi.nlm.nih.gov/pubmed/19908201
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.1731
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