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Purification of proteins containing zinc finger domains using Immobilized Metal Ion Affinity Chromatography
Heterologous proteins are frequently purified by Immobilized Metal Ion Affinity Chromatography (IMAC) based on their modification with a hexa-histidine affinity tag (His-tag). The terminal His-tag can, however, alter functional properties of the tagged protein. Numerous strategies for the tag remova...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3134162/ https://ncbi.nlm.nih.gov/pubmed/21600288 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2011.04.022 |
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