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Purification of Hemoglobin from Red Blood Cells using Tangential Flow Filtration and Immobilized Metal Ion Affinity Chromatography

Two methods for purifying hemoglobin (Hb) from red blood cells (RBCs) are examined and compared. In the first method, red blood cell lysate is clarified with a 50 nm tangential flow filter and hemoglobin is purified using immobilized metal ion affinity chromatography (IMAC). In the second method, RB...

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Bibliografske podrobnosti
Main Authors: Elmer, Jacob, Harris, David, Palmer, Andre F.
Format: Artigo
Jezik:Inglês
Izdano: 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3023821/
https://ncbi.nlm.nih.gov/pubmed/21195679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2010.11.021
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