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Purification of Hemoglobin from Red Blood Cells using Tangential Flow Filtration and Immobilized Metal Ion Affinity Chromatography

Two methods for purifying hemoglobin (Hb) from red blood cells (RBCs) are examined and compared. In the first method, red blood cell lysate is clarified with a 50 nm tangential flow filter and hemoglobin is purified using immobilized metal ion affinity chromatography (IMAC). In the second method, RB...

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書誌詳細
主要な著者: Elmer, Jacob, Harris, David, Palmer, Andre F.
フォーマット: Artigo
言語:Inglês
出版事項: 2010
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3023821/
https://ncbi.nlm.nih.gov/pubmed/21195679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2010.11.021
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