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Purification of Squash NADH:Nitrate Reductase by Zinc Chelate Affinity Chromatography

NADH:nitrate reductase (EC 1.6.6.1) was isolated and purified from the green cotyledons of 5-day-old squash seedlings (Cucurbita maxima L.). The 10-hour purification procedure consisted of two steps: direct application of crude enzyme to blue Sepharose and specific elution with NADH followed by dire...

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Bibliografski detalji
Glavni autori: Redinbaugh, Margaret G., Campbell, Wilbur H.
Format: Artigo
Jezik:Inglês
Izdano: 1983
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1067205/
https://ncbi.nlm.nih.gov/pubmed/16662789
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