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Semiquantitative Proteomic Analysis of the Human Spliceosome via a Novel Two-Dimensional Gel Electrophoresis Method

More than 200 proteins associate with human spliceosomes, but little is known about their relative abundances in a given spliceosomal complex. Here we describe a novel two-dimensional (2D) electrophoresis method that allows separation of high-molecular-mass proteins without in-gel precipitation and...

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Bibliografski detalji
Glavni autori: Agafonov, Dmitry E., Deckert, Jochen, Wolf, Elmar, Odenwälder, Peter, Bessonov, Sergey, Will, Cindy L., Urlaub, Henning, Lührmann, Reinhard
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3133382/
https://ncbi.nlm.nih.gov/pubmed/21536652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.05266-11
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