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Characterization of purified human B(act) spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis
To better understand the compositional and structural dynamics of the human spliceosome during its activation, we set out to isolate spliceosomal complexes formed after precatalytic B but prior to catalytically active C complexes. By shortening the polypyrimidine tract of the PM5 pre-mRNA, which lac...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2995400/ https://ncbi.nlm.nih.gov/pubmed/20980672 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2456210 |
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