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Mass spectrometry–based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count

The spliceosome undergoes major changes in protein and RNA composition during pre-mRNA splicing. Knowing the proteins—and their respective quantities—at each spliceosomal assembly stage is critical for understanding the molecular mechanisms and regulation of splicing. Here, we applied three independ...

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Hlavní autoři: Schmidt, Carla, Grønborg, Mads, Deckert, Jochen, Bessonov, Sergey, Conrad, Thomas, Lührmann, Reinhard, Urlaub, Henning
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3923134/
https://ncbi.nlm.nih.gov/pubmed/24448447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.041244.113
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