Assembly of large genomic segments in artificial chromosomes by homologous recombination in Escherichia coli
We developed a method for the reconstruction of a 100 kb DNA fragment into a bacterial artificial chromosome (BAC). The procedure makes use of iterative rounds of homologous recombination in Escherichia coli. Smaller, overlapping fragments of cloned DNA, such as cosmid clones, are required. They are...
Na minha lista:
| 發表在: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
2001
|
| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC31298/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11266572/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.7.e37 |
| 標簽: |
沒有標簽, 成為第一個標記此記錄!
|