Assembly of large genomic segments in artificial chromosomes by homologous recombination in Escherichia coli

We developed a method for the reconstruction of a 100 kb DNA fragment into a bacterial artificial chromosome (BAC). The procedure makes use of iterative rounds of homologous recombination in Escherichia coli. Smaller, overlapping fragments of cloned DNA, such as cosmid clones, are required. They are...

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Podrobná bibliografie

Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Sosio, Margherita, Bossi, Elena, Donadio, Stefano
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2001
Témata:	NAR Methods Online
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC31298/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11266572/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.7.e37
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