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Unamplified cap analysis of gene expression on a single-molecule sequencer
We report the development of a simplified cap analysis of gene expression (CAGE) protocol adapted for single-molecule sequencers that avoids second strand synthesis, ligation, digestion, and PCR. HeliScopeCAGE directly sequences the 3′ end of cap trapped first-strand cDNAs. As with previous versions...
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| Главные авторы: | , , , , , , , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Cold Spring Harbor Laboratory Press
2011
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3129257/ https://ncbi.nlm.nih.gov/pubmed/21596820 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.115469.110 |
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