Unamplified cap analysis of gene expression on a single-molecule sequencer

We report the development of a simplified cap analysis of gene expression (CAGE) protocol adapted for single-molecule sequencers that avoids second strand synthesis, ligation, digestion, and PCR. HeliScopeCAGE directly sequences the 3′ end of cap trapped first-strand cDNAs. As with previous versions...

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Bibliografiske detaljer
Main Authors: Kanamori-Katayama, Mutsumi, Itoh, Masayoshi, Kawaji, Hideya, Lassmann, Timo, Katayama, Shintaro, Kojima, Miki, Bertin, Nicolas, Kaiho, Ai, Ninomiya, Noriko, Daub, Carsten O., Carninci, Piero, Forrest, Alistair R.R., Hayashizaki, Yoshihide
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2011
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Method
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3129257/
https://ncbi.nlm.nih.gov/pubmed/21596820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.115469.110
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