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Topoisomerase IV, alone, unknots DNA in E. coli
Knotted DNA has potentially devastating effects on cells. By using two site-specific recombination systems, we tied all biologically significant simple DNA knots in Escherichia coli. When topoisomerase IV activity was blocked, either with a drug or in a temperature-sensitive mutant, the knotted reco...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC312656/ https://ncbi.nlm.nih.gov/pubmed/11274059 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.872301 |
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