Purification and characterization of TnsC, a Tn7 transposition protein that binds ATP and DNA.
The bacterial transposon Tn7 encodes five transposition genes tnsABCDE. We report a simple and rapid procedure for the purification of TnsC protein. We show that purified TnsC is active in and required for Tn7 transposition in a cell-free recombination system. This finding demonstrates that TnsC par...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1992
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312388/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1317955/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.10.2525 |
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